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J.Health Sci., 57(5), 401-405, 2011
-Regular Article-
Effects of a Purified Saponin Mixture from Alfalfa on Plasma Lipid Metabolism in Hyperlipidemic Mice
Cong-Hong Yu,#, a Guo Xie,#, b, c
Rong-Rong He,c Yu-Jia Zhai,c
Yi-Fang Li,c Bun Tsoi,c
Hiroshi Kurihara,c and De-Po Yang*, a
aSchool of Pharmaceutical Sciences, Sun Yat-sen University, No. 132, East Outer Ring Road, Guangzhou Higher Education Mega Center, Guangzhou 510275, China,
bZhongshan Institute, University of Electronic Science and Technology of China, No. 1, College Road, Shiqi District, Zhongshan 528402, China and
cInstitute of Traditional Chinese Medicine and Natural Products, Jinan University, No. 601, West of Huangpu street, Guangzhou 510632, China
Alfalfa (Medicago sativa L.) is a genus distributed widely throughout the world and considered as the richest land source of nature products. In this study, we obtained a purified saponin mixture (PSM) through a simple, repeatable and controllable separation method from alfalfa. The compounds of the saponin mixture were identified as Azukisaponin V and Soyasaponin I, which was determined as 79.26 and 20.74% respectively by HPLC-evaporative light scattering detection (ELSD) method. The effects of PSM on plasma lipid metabolism were studied in hyper-lipidemic mice induced by Triton WR-1339. Results showed that PSM could significantly inhibit the increase of total cholesterol (TC), triacylglycerol (TG) and low density lipoprotein cholesterol (LDL-C) levels, and the decrease of plasma high density lipoprotein cholesterol (HDL-C) level in plasma of hyperlipidemic mice induced by Triton WR-1339. Further study demonstrated that PSM not only enhanced the activities of lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) in liver, but also increased their mRNA levels in hyperlipidemic mice. The results above indicate that PSM from alfalfa exerts effects on plasma lipid metabolism through elevating the activity of liver lipases in hyperlipidemic mice. The obtained data would be a good contribution to the development and utilization of alfalfa in the world.
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