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J.Health Sci., 56(6), 727-732, 2010
-Research Letter-
Phosphoenolpyruvic Acid, an Intermediate of Glycolysis, Attenuates Cellular Injury Induced by Hydrogen Peroxide and 2-Deoxy-D-glucose in the Porcine Proximal Kidney Tubular Cell Line, LLC-PK1
Yuki Kondo,a Yoichi Ishitsuka,*, a
Daisuke Kadowaki,b, c Minako Nagatome,a
Yusuke Saisho,a Masataka Kuroda,a
Sumio Hirata,c Mitsuru Irikura,a
Naotaka Hamasaki,d and Tetsumi Iriea, c
aDepartment of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Kumamoto 862-0973, Japan,
bDepartment of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Kumamoto 862-0973, Japan,
cCenter for Clinical Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Kumamoto 862-0973, Japan and
dDepartment of Clinical Chemistry and Laboratory Medicine, Nagasaki International University, Sasebo 859-3298, Japan
This study was conducted to clarify whether phosphoenolpyruvate (PEP), an intermediate substance of glycolysis, has the potential to attenuate cellular injury induced by oxidative stress or dysfunctions in energy metabolism in vitro. PEP (0.5-10 mM) attenuated hydrogen peroxide (H2O2)-induced cellular injury in the porcine proximal kidney tubular cell line, LLC-PK1 in a dose-dependent manner. PEP also prevented cellular injury in LLC-PK1 cells induced by the glycolysis inhibitor, 2-deoxy-D-glucose (2-DG). In addition, PEP significantly enhanced the degradation of H2O2. The prevention of H2O2-induced cellular injury mediated by PEP was more potent than that of the carbohydrates, glucose and trehalose, which are used as components of organ preservation solutions for clinical transplantation. In conclusion, we demonstrated that PEP is a bifunctional carbohydrate with anti-oxidant properties and suggest that PEP is potentially useful as an organ preservation agent in clinical transplantation.
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