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J.Health Sci., 56(5), 598-605, 2010
-Regular Article-
Monolithic Spin-column Extraction and GC-MS Method for the Assay of Eperisone in Human Serum
Takeshi Saito,*, a Takeshi Yamagiwa,a
Yuko Yui,b Shota Miyazaki,b
Akihiro Nakamoto,c, d Akira Namera,d
and Sadaki Inokuchia
aDepartment of Emergency and Critical Care Medicine, Tokai University School of Medicine, Shimokasuya 143, Isehara 259-1143, Japan,
bGL Sciences Inc., Sayamagahara 237-2, Iruma 358-0032, Japan,
cScientific Investigation Laboratory, Hiroshima Prefectural Police Headquarters, Kohnan 2-26-3, Naka-ku, Hiroshima 730-0825, Japan and
dDepartment of Legal Medicine, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8551, Japan
A rapid gas chromatography-mass spectrometry (GC-MS) method was developed and validated for the analysis of eperisone in serum using monolithic spin-column extraction. The linear concentration range for eperisone was 2-2500 ng/ml. The limit of detection was found to be 0.5 ng/ml. The average extraction recovery range was 92.8-96.0%. The intra- and interday relative standard deviations (RSDs) of the concentrations were less than 12.6% and 12.5%, respectively. The accuracy of this method ranged from 95.0% to 98.3%. We successfully used this assay to analyze serum samples from an eperisone-overdose patient. Our method has some analytical advantages compared with previously reported gas chromatography (GC) and GC-MS methods, such as higher selectivity and sensitivity than GC with nitrogen-phosphorus detection and the avoidance of nonspecificity, the ability to use a smaller sample volume than that required for the GC-MS method, and a shorter sample preparation time than the previous solid-phase extraction (SPE) method.
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