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J.Health Sci., 56(3), 321-325, 2010
-Research Letter-
Oligonucleotide Probes for Phylogenetic Detection of Waterborne Bacteria
Tomoaki Ichijo,a Nobuyasu Yamaguchi,a
Katsuji Tani,b and Masao Nasu*, a
aGraduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamada-oka, Suita, Osaka 565-0871, Japan and
bSchool of Pharmacy, Osaka Ohtani University, 3-11-1, Nishikiori-kita, Tondabayashi, Osaka 584-8540, Japan
Waterborne diseases were globally occurred, and many people were suffered from and often killed by them. In order to prevent outbreaks of waterborne diseases, rapid detection of pathogenic microbes in aquatic environment is the important strategy in addition to the construction of water supply and vaccination. The 16S ribosomal RNA (rRNA) gene is often used as a target gene for bacterial detection using hybridization techniques. In this study, we aimed to design the oligonucleotide probes that could be used for the detection of waterborne bacteria with hybridization techniques because design of specific probes is important to assure for the precise detection of target bacteria. We then evaluated the specificities of designed probes by using an oligonucleotide microarray. In conclusion, we confirmed that seven designed oligonucleotide probes were suitable for the specific detection of waterborne bacteria. These probes appear to be used for 16S rRNA targeted hybridization techniques such as fluorescence in situ hybridization (FISH) and oligonucleotide microarray.
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