| |
Software Requirements
Microsoft Internet Explorer 5.01 or higher and Netscape Navigator 4.75 or higher are recommended. |
|
J.Health Sci., 55(6), 939-945, 2009
Comparison of the Solid Phase-supported Receptor and Ligand Competitive Assays for Biotin
Makoto Muratsugu,* Namiko Amano,
and Rie Takeda
Bioanalytical Science Laboratory, Department of Clinical Nutrition, Osaka Prefecture University, 3-7-30 Habikino, Habikino, Osaka 583-8555, Japan
Solid phase-supported receptor and ligand competitive assays for biotin were tested with respect to a mathematical model. We could assume the same mathematical model for both the solid phase-supported receptor and ligand competitive assays. In addition, we could eliminate the so-called blocking procedure in these assays. The amount of immobilizing reagent (biotinylated bovine serum albumin, BBSA) in the solid phase-supported ligand competitive assay was less than that (avidin) in the solid phase-supported receptor competitive assay. Desthiobiotin and biocytin influenced the solid phase-supported ligand competitive assay, but lipoic acid and lysine did not.
|
|
