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J.Health Sci., 55(4), 578-585, 2009
Electron Microscopic Study on Lysis of a Cyanobacterium Microcystis
Keiko Ozaki,a Emiko Ito,b
Saori Tanabe,a Kaori Natsume,a
Kiyomi Tsujic, and Ken-ichi Harada*, a
aGraduate School of Environmental and Human Science, and Faculty of Pharmacy, Meijo University, Yagotoyama 150 Tempaku, Nagoya 468-8503, Japan,
bResearch Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Inohana 1-8-1 Chuoku, Chiba 260-8673, Japan and
cKanagawa Prefectural Institute of Public Health, Shimomachiya, Shimomachiya 1-3-1 Chigasaki, Kanagawa 253-0087, Japan
In a previous study, it was suggested that β-cyclocitral plays an important role in understanding the lysis of cyanobacteria under natural conditions. The present study was conducted in order to understand how the β-cyclocitral lyses cyanobacterial cells, and other anticyanobacterial agents were also investigated. The preliminary study using a scanning electron microscope (SEM) demonstrated that there were three types of morphological changes in the cyanobacterial cells when they were incubated with anticyanobacterial agents: volatile compounds from cyanobacteria cause shrinking and then wrinkling; terpenoids contact directly and cause stripping; basic amino acids cause swelling and then collapsing. In order to clarify the extreme difference in the damage during the morphological changes between the β-cyclocitral and L-lysine(Lys), the transmission electron microscope (TEM) technique was applied. Although a definite difference in the morphological damage was observed, a plausible mechanism for β-cyclocitral could be not deduced. Throughout the experiments using antibiotics, it was found that the apparent morphological changes after lysis did not always correspond to the mode of action.
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