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J.Health Sci., 55(2), 169-177, 2009
The Effect of Generation 2 and 3 Poly (amidoamine) Dendrimers on Viability of Human Breast Cancer Cells
Katarzyna Winnicka,*, a Krzysztof Bielawski,b
Magorzata Rusak,c and Anna Bielawskab
aDepartment of Pharmaceutical Technology,
bDepartment of Medicinal Chemistry and Drug Technology,
cDepartment of Hematological Diagnostics, Faculty of Pharmacy, Medical University of Bialystok, Kilinskiego 1, 15-089 Bialystok, Poland
In this study, we investigated the effect of amine- and hydroxyl-terminated Generation 2 and 3 (G2 and G3) poly (amidoamine) (PAMAM) dendrimers on the viability and induction of apoptosis in human MCF-7 and MDAMB-231 breast cancer cells. It was shown that amino-terminated PAMAM dendrimers were cytotoxic, with IC50 value after 24 hr of incubation in MCF-7 and MDA-MB-231 breast cancer cells 153±3 μM and 140±2 μM for G2 PAMAM and 120±3 μM and 99±2 μM for G3 PAMAM, respectively. Experiments made with annexin V-fluorescein isothiocyanate (V-FITC) and detection of apoptosis by a fluorescent microscopy assay revealed that G2 and G3 PAMAM-NH2 dendrimers inhibited the proliferation of MCF-7 and MDA-MB-231 malignant cells by increasing the number of apoptotic and necrotic cells. The cytotoxic and apoptotic effect of G2 and G3 PAMAMOH dendrimers was significantly weaker.
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