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J.Health Sci., 54(6), 692-696, 2008
Cytometric Analysis of the Cytotoxic Action of Adenosine 5'-Monophosphate on Rat Thymocytes
Yoko Sakanashi,a Midori Morimoto,a
Yumiko Nishimura,b Hiroko Matsui,c
Kaori Kanemaru,a Toshiya Masuda,a
and Yasuo Oyamaa, *
aLaboratories of Cellular Signaling and Bioorganic Chemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima, Minami-Jyosanjima 1-1, Tokushima 770-8502,
bOkayama University Dental School, Shikada 2-5-1, Okayama 700-8525, and
cFaculty of Dental Science, Kyushu University, Maidashi 3-1-1, Fukuoka 812-8582, Japan
The cascade of adenosine 5'-monophosphateactivated protein kinase (AMPK) is known to be a sensor of cellular energy charge. In this context, a paradigm for obesity treatment via the activation of AMPK has attracted the suppliers of complementary medicines and supplements. It is possible that products that increase the concentration of adenosine 5'-monophosphate (AMP) in the body would be efficacious in reducing body weight. However, since there is currently little information concerning the toxicity of AMP, the cytotoxic action of AMP on rat thymocytes was examined by flow cytometry. Incubation of cells with AMP at a concentration of 30 μM or more for 24hr significantly increased the populations of dead cells, shrunken cells, and cells containing hypodiploidal DNA in a concentration-dependent manner. Z-VAD-FMK, a pan-inhibitor of caspases, attenuated the AMP-induced changes in cell populations. It is concluded that AMP at a concentration of 30 μM or more exerts a cytotoxic action, which is dependent on the activation of caspases.
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