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J.Health Sci., 54(5), 602-606, 2008
A Fast and Simple Analysis of Glyphosate in Tea Beverages by Capillary Electrophoresis with On-Line Copper(II)-Glyphosate Complex Formation
Shuji Kodama,*, a Yuji Ito,b
Atsushi Taga,c Yoko Nomura,d
Atsushi Yamamoto,d Satoshi Chinaka,e
Kentaro Suzuki,f Tomohisa Yamashita,a
Tomoko Kemmei,a and Kazuichi Hayakawag
aToyama Institute of Health, 17-1 Nakataikoyama, Imizu, Toyama 939-0363, Japan,
bKirin Holdings Co. Ltd., 3 Miyahara, Takasaki, Gunma, 370-1295, Japan,
cSchool of Pharmacy, Kinki University, 3-4-1 Kowakae, Higashi-Osaka 577-8502, Japan,
dDepartment of Biological Chemistry, College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai-shi, Aichi 487-8501, Japan,
eForensic Science Laboratory, Ishikawa Prefectural Police Headquarters, 1-1 Kuratsuki, Kanazawa 920-8553, Japan,
fFukushima Factory, GL Sciences Inc., 5-3 Nagasone, Okajima, Fukushima 960-8201, Japan, and
gGraduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan
Glyphosate is an herbicide used for many plants that can be toxic to humans in high doses. Current methods for measuring glyphosate are slow and expensive. Here we describe a fast and simple method for measuring glyphosate in tea beverages by capillary electrophoresis with on-line formation of copper(II)-glyphosate complex. The optimum running conditions were found to be 40 mM acetate buffer (pH 5.0) containing 5 mM CuSO4 with an effective voltage of +15 kV using a sulfonated capillary (FunCap-CE Type S) and direct UV detection at 250 nm. Linearity (r2>0.999) was demonstrated in the range 5-1000 mg/l of glyphosate. Good reproducibilities of peak area (relative standard deviation <1.2%) and migration time (relative standard deviation <0.2%) were obtained. Recovery of glyphosate was between 98 and 100%. With this method, a tea beverage that was mixed with a glyphosate formulation was successfully analyzed.
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