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J.Health Sci., 54(1), 43-49, 2008
Essential Role of Ascorbic Acid in Neural Differentiation and Development: High Levels of Ascorbic Acid 2-Glucoside Effectively Enhance Nerve Growth Factor-Induced Neurite Formation and Elongation in PC12 Cells
Mari Haramoto,a Hideki Tatemoto,b
and Norio Muto*, a
aGraduate School of Comprehensive Scientific Research, Program in Biological System Sciences, Prefectural University of Hiroshima, 562 Nanatsuka, Shobara 727-0023, Japan and
bFaculty of Agriculture, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan
The aim of this study was to investigate the role of ascorbic acid (AsA) in neural differentiation induced by nerve growth factor (NGF) in PC12 cells, a neural precursor cell line. Cells were cultivated in the presence of L-ascorbic acid-2-O-α-D-glucopyranoside (AA-2G) and evaluated for potentiation of neural differentiation induced by a small amount of NGF. AA-2G enhanced the proportion of neurite-bearing cells in a concentration-dependent manner (<1 mM). When cultivated with relatively high concentrations of AA-2G (1-5 mM), the cells tended to extend neurites further and to form a neuron-like network. The resultant number of neurite-bearing cells was apparently increased by the addition of AA-2G, with a concurrent decrease in the number of total cells in the same culture dish. These results suggest that AsA in the brain not only promotes NGF-induced differentiation of neural precursor cells but also participates in the development of neural network-forming cells.
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