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J.Health Sci., 53(4), 500-505, 2007
Potential Anti-inflammatory Properties of Crude Alcoholic Extract of Ocimum basilicum L. in Human Peripheral Blood Mononuclear Cells
Chinnasamy Selvakkumar,a Balakrishnan Gayathri,a
Kontham Sanathkumar Vinaykumar,a Baddireddi Subhadra Lakshmi,*, a
and Arun Balakrishnanb
aCentre for Biotechnology, Anna University, Chennai, Tamil Nadu 600 025, India and
bNicholas Piramal Research Centre, 1, Nirlon complex, Off Western Express Highway, Goregaon East (E), Mumbai-400 063, India
Ocimum basilicum L. (Lamiaceae) is a well-known perennial herb in Indian medicine used to treat various disorders like upper respiratory tract infections and wound healing. Primarily we investigated the anti-inflammatory activity of crude extracts of Ocimum basilicum using peripheral blood mononuclear cells (PBMC) of healthy individuals. Ocimum basilicum crude methanolic extract showed a good inhibitory effect on the proliferative response of PBMC in mitogenic lymphocyte proliferation assays. Furthermore, gene expression studies on lipopolysaccharide (LPS) induced production of proinflammatory cytokines like Tumor necrosis factor-α (TNF-α), Interleukin-1β (IL-1β) and IL-2 showed down regulation of the markers. It also suppressed the induction of inducible nitric oxide synthase (iNOS) and the subsequent production of nitric oxide (NO) in LPS-stimulated RAW 264.7 macrophages in a time-dependent manner. Our results showed that Ocimum basilicum crude methanolic extract inhibits the key proinflammatory cytokines and mediators, which accounts for its anti-inflammatory effect.
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