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J.Health Sci., 53(4), 491-495, 2007
Usefulness of Catechins and Caffeine Profiles to Determine Growing Areas of Green Tea Leaves of a Single Variety, Yabukita, in Japan
Shuji Kodama,*, a Yuji Ito,b
Hirofumi Nagase,a Tomohisa Yamashita,a
Tomoko Kemmei,a Atsushi Yamamoto,c
and Kazuichi Hayakawad
aToyama Institute of Health, 17-1 Nakataikoyama, Imizu, Toyama 939-0363, Japan,
bKirin Brewery Co. Ltd., 3 Miyahara, Takasaki, Gunma, 370-1295, Japan,
cDepartment of Biological Chemistry, College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai-shi, Aichi 487-8501, Japan, and
dGraduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan
Yabukita green tea leaves in Shizuoka, Kagoshima and Mie areas in Japan were analyzed for catechins and caffeine using 6-O-α-D-glucosyl-β-cyclodextrin-modified micellar electrokinetic chromatography (6G-β-CD-modified MEKC). Caffeine and seven catechins [(-)-catechin (C), (+)-C, (-)-epicatechin (EC), (-)-catechin gallate (CG), (-)-epicatechin gallate (ECG), (-)-epigallocatechin (EGC) and (-)-epigallocatechin gallate (EGCG)], but not (+)-EC, were detected in all extracts. Linear discriminant analysis was applied to differentiate green tea samples according to their geographical origins. As a result, all samples were correctly classified, which represented a recognition ability of 100%. These results suggest that catechins and caffeine profiles of Yabukita green tea leaves could be useful to determine their growing areas in Japan.
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