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J.Health Sci., 52(3), 268-275, 2006
Inhibitory Effects of Bee Pollen Cistus ladaniferus Extract on Bone Resorption in Femoral Tissues and Osteoclast-Like Cell Formation in Bone Marrow Cells in Vitro
Reiko Hamamoto,a Kaori Ishiyama,b and Masayoshi Yamaguchi*, a
aLaboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan and bInstitute for Health Science, Yamada Apiculture Center, Inc., 194 Ichiba, Kagamino-cho, Tomata-gun, Okayama-ken 708-0393, Japan
The effects of bee pollen extract on osteoclastic bone resorption in vitro were investigated. The water-solubilized extracts were obtained from the bee pollen of Cistus ladaniferus. Femoral-diaphyseal or -metaphyseal tissues of rats were cultured for 48 hr in medium containing either vehicle, bone-resorbing factor, or bone-resorbing factor plus bee pollen extracts (10, 100, or 1000 mu g/ml of medium). Culture with parathyroid hormone [human (1-34) PTH; 10-7 M], prostaglandin E2 (PGE2; 10-5 M), or 1,25-dihydroxyvitamin D3 (10-6 M) caused a significant decrease in calcium content in the diaphyseal or metaphyseal tissues. These decreases were completely prevented in the presence of bee pollen extracts (10, 100, or 1000 mu g/ml). The presence of PTH (10-7 M) caused a significant increase in medium glucose consumption and lactic acid production in the diaphyseal or metaphyseal tissues. These increases were significantly inhibited by culture with bee pollen extracts (10, 100, or 1000 mu g/ml). Mouse bone marrow cells were cultured for 7 days in the presence of bone-resorbing factor in vitro. Culture with PTH (10-7 M), PGE2 (10-5 M), tumor necrosis factor-alpha(10 ng/ml of medium), or lypopolysaccharide (10 mu g/ml of medium) caused a significant increase in osteoclast-like cell formation. These increases were significantly inhibited in the presence of bee pollen extracts (10, 50, or 100 mu g/ml of medium). This study demonstrates that bee pollen extract has inhibitory effects on osteoclastic bone resorption in vitro.
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