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J.Health Sci., 52(1), 78-81, 2006
Identification Tests of Aristolochic Acid in Crude Drugs by Reversed-Phase TLC/Scanning Densitometry
Tsutomu Ohno,*, a Eiichi Mikami,a Hiroshi Matsumoto,a and Naoki Kawaguchib
aAichi Prefectural Institute of Public Health, 7-6 Nagare, Tsuji-machi, Kita-ku, Nagoya 462-8576, Japan and bFaculty of Pharmacy, Meijo University, 150 Yagotoyama, Tempaku-ku, Nagoya 468-8503, Japan
We performed identification tests of aristolochic acid in Xixin, Mutong, Muxiang, and Fangji by reversed-phase TLC/scanning densitometry using aristolochic acid I as a marker compound. The developing solvents used were: I) acetonitrile/methanol/water mixture solution (3 : 1 : 1) and II) 2-butanone/methanol/sodium sulfate(1→20) mixture solution (2 : 1 : 1). The Rf value of aristolochic acid I was 0.54 using the I system and 0.57 using the II system. The maximum absorption wavelengths of aristolochic acid I observed by scanning densitometry were 254 and 325 nm.
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