| |
Software Requirements
Microsoft Internet Explorer 5.01 or higher and Netscape Navigator 4.75 or higher are recommended. |
|
 |
J.Health Sci., 51(4), 483-487, 2005
Identification and Determination of Cannabinoids in both Commercially Available and Cannabis Oils Stored Long Term
Mamoru Yotoriyama,*, a Eiji Ishiharajima,a Yoko Kato,a Akiko Nagato,a Setsuko Sekita,b Kazuhito Watanabe,c and Ikuo Yamamotod
aTochigi Prefectural Institute of Public Health and Environment, 2145-13 Shimookamoto, Kawachi-machi, Tochigi 329-1196, Japan, bTsukuba Medicinal Plant Research Station, National Institute of Health Sciences, 1 Hachimandai, Tsukuba-city, Ibaraki 305-0843, Japan, cFaculty of Pharmaceutical Sciences, Hokuriku University, Ho-3, Kanagawa-machi, Kanazawa 920-1181, Japan, and dSchool of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshino-machi, Nobeoka-city, Miyazaki 882-8508, Japan
Among cannabinoids (CNs) in two commercially available and long-term stored cannabis oils obtained from the seeds of Cannabis sativa L. in Japan, tetrahydrocannabinol (THC), canabidiol (CBD), cannabinol (CBN), and cannabichromene were identified and determined using high-performance thin-layer chromatography and capillary gas chromatography-mass speectrometry after partitioning extraction with n-hexane/acetonitrile. CNs were determined in two commercially available cannabis oils, designated "Hemp oil" (THC, 16.3 mu g/g and CBN, 5.9 mu g/g) and "Taima-yu" (CBD, 26.1 mu g/g). The concentration of CBD in a cannabis oil that had been stored for 20 years was 107 ± 4 mu g/g. However, no other CNs including THC were detected in the oil. The CBD content in the original oil was estimated to be about 130 mu g/g from the determination values of the two periods. These results suggest that cannabis oils generally contain CNs to some extent.
|
|
