| |
Software Requirements
Microsoft Internet Explorer 5.01 or higher and Netscape Navigator 4.75 or higher are recommended. |
|
 |
J.Health Sci., 51(3), 341-345, 2005
Apoptosis Induced in Human Cell Lines by a Butanol Extract from Chlorophytum comosum Roots
Hirohisa Matsushita,*, a, b Hiroaki Kuwabara,b Satoko Ishikawa,b and Masataka Mochizukib
aBioscience Research & Development Center, Nichirei, Kumegawa-chou 1-52-14, Higashimurayama-shi, Tokyo 189-0003, Japan and bDivision of Organic and Bioorganic Chemistry, Kyoritsu University of Pharmacy, Shibakoen 1-5-30, Minato-ku, Tokyo 105-8512, Japan
The antiproliferative effects of a n-butanol extract from Chlorophytum comosum (C. comosum) (spider plant) roots was tested in vitro against four human cell lines: HeLa, CCRF-HSB-2, HL-60 and U937 cells. The extract was found to have cell antiproliferative effects against HeLa, CCRF-HSB-2, HL-60 and U937 cell lines, with an IC50 value of 6.6, 5.4, 8.7 and 10.2 mu g/ml, respectively. The dying cells showed characteristics of apoptosis, such as DNA fragmentation, as assessed by DNA electrophoresis analysis and the Terminal deoxynucleotidyl transferase (TdT)-mediated biotin dUTP Nick End-Labeling (TUNEL) method. These results indicate that extract from C. comosum roots can induce apoptosis in human cell lines.
|
|
