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J.Health Sci., 51(1), 96-100, 2005
High Temperature Condition Enhances Retrovirus Infection to Cell
Akira Tempaku*
School of Medicine, Faculty of Medical Sciences, University of Fukui, 23-3 Shimoaizuki, Matsuoka-cho, Yoshida-gun, Fukui 910-1193, Japan
The amount of retrovirus infection depended on the temperature during the virus entry period. Higher amount of retrovirus [human immunodeficiency virus type-1 (HIV-1) and murine leukemia virus] infected to cell at 40°C than at 37°C. One-hour adsorption at 40°C marked more than 10 times large amount of infection than that at 37°C. This phenomenon is observed in both X4 and R5 type HIV-1 strains. At 40°C condition, more than four times much provirus DNA was accumulated in cell than that at 37°C condition, while same amount of virus adsorption was observed in both at 40 and 37°C adsorption. Similar enhancement was observed in murine leukemia virus infection, while little effect was monitored in rhabdovirus (VSV) infection. This result said that high temperature condition prompted the retrovirus penetration (membrane fusion mediated way) to cell. This observation would applicable to improve the gene transduction efficiency using retrovirus vector.
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