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J.Health Sci., 51(1), 93-95, 2005
Estrogenic Potency of a Bisphenol A Metabolite on Vitellogenin Synthesis in Medaka, Oryzias latipes
Masaki Nagae,*, a Ken'ichirou Shiroyama,a Mihoka Inoue,c Akihiko Hara,c Yuji Takao,a Shinya Kohra,a Yasuhiro Ishibashi,b Nobuaki Tominaga,d Shin'ichi Yoshihara,e and Koji Arizonof
aFaculty of Environmental Studies and bEnvironmental Protection Center, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan, cDivision of Marine Bioscience, Graduate School of Fisheries Science, 3-1-1 Minatochou, Hakodate 041-0802, Japan, dDepartment of Chemical and Biological Engineering, Ariake National College of Technology, 150 Higashihagino-machi, Omuta 836-8585, Japan, eDepartment of Xenobiotic Metabolism and Molecular Toxicology, Graduate School of Biomedical Science, Hiroshima University, 1-2-3 Kasumi, Hiroshima 734-8851, Japan, and fFaculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, Tsukide 3-1-100, Kumamoto 862-8502, Japan
We investigated the estrogenic activity of a bisphenol A (BPA) metabolite (4-methyl-2,4-bis(p-hydroxyphenyl)-pent-1-ene; MBP) in male medaka (Oryzias latipes) using vitellogenin (Vg, Vg1 and Vg2) as a biomarker. Male d-rR medaka were exposed to various concentrations of estradiol-17beta (E2), MBP and BPA for 3 days, and then the serum Vg concentration was measured using specific chemiluminescent immunoassays. The estimated relative estrogenic activities of MBP and BPA compared with E2 (100%) were 1.3-1.4% and 0.00010-0.00023%, respectively. These findings indicated that MBP has about 104-fold higher estrogenic potency than the parent BPA and about 1/50 that of E2 for Vg synthesis in medaka. This is the first study to show that MBP can act as a highly potent estrogen agonist in living organisms.
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