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J.Health Sci., 50(6), 685-688, 2004
Evaluation of Ability of Chemicals to Bind Frog
(Xenopus laevis)
Estrogen Receptor
by in Vitro Binding
Assay
Eiko Suzuki, Masaaki Kunimoto,
Makoto Nishizuka,
and Masayoshi Imagawa*
Department of Molecular Biology, Graduate School of
Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori,
Mizuho-ku, Nagoya, Aichi 467-8603, Japan
The in vitro binding assay seems to be a useful first
screening method for endocrine disrupting chemicals.
Various methods have been developed and applied to
the testing of chemicals. Since these assays should be
applied to estrogen receptors (ER) of not only humans
but also wildlife, we previously developed a
standardized in vitro binding assay system for human, quail,
Japanese medaka, and Xenopus laevis ERs using a
competitive enzyme immunoassay. Since that was a first
report on an in vitro binding assay system for
Xenopus ER alpha, and the capacities of chemicals to bind
Xenopus ER alpha were not tested yet, we here evaluated the ability
of 20 test chemicals, which were selected by the
Ministry of the Environment of Japan, to bind
Xenopus ER alpha. Of these, 4-nonylphenol,
p-octylphenol and bisphenol A had relatively high binding capacity, and these
results are similar to those obtained previously in quail
ER alpha.
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