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J.Health Sci., 50(6), 679-684, 2004
Dose-Response Study of Daily Cocoa Intake on the
Oxidative Susceptibility of Low-Density Lipoprotein in
Healthy Human Volunteers
Naomi Osakabe,*, a Seigo
Baba,a
Akiko Yasuda,a Tamami
Iwamoto,b
Masumi Kamiyama,b Takahisa
Tokunaga,a and Kazuo
Kondob
aHealth and Bioscience Laboratories, Meiji Seika Kaisha Ltd.,
5-3-1, Chiyoda Sakado, Saitama 350-0289, Japan and
bInstitute of Environmental Science for Human Life,
Ochanomozu University, 2-1-1, Ohtsuka, Bunkyo-ku, Tokyo
112-8610, Japan
A four-period crossover study was conducted to
evaluate the dose-related response of daily cocoa
intake on the oxidative susceptibility of low-density
lipoprotein (LDL) in healthy human volunteers. Each
supplementation phase consisted of a 14-day feeding
period followed by a 28-day washout period. During
the period, healthy male volunteers (n = 8) ingested 18,
24 or 36 g of cocoa powder per day (1.3, 1.74 or 2.61 g
of polyphenols per day). During the control period,
these subjects ingested sugar. LDL oxidative
susceptibility was measured as the lag time of conjugated
diene formation that started with the addition of a
radical initiator, 2,2'-azobis
(4-methoxy-2,4-dimethylvaleronitrile). Samples were analyzed at pre-, one week,
and two weeks post-supplementation. In the 24 and 36 g cocoa powder ingestion period, significant lag time
prolongation was observed. We conclude that the
ingestion of more than 24 g of cocoa powder per day
(1.74 g of polyphenols) clearly protects LDL from
oxidation.
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