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J.Health Sci., 50(6), 660-665, 2004
Disaccharide Composition of Glycosaminoglycan Chains
in Growing Vascular Endothelial Cells in
Culture after Exposure
to Lead
Yasuyuki Fujiwara,a Toshiyuki
Kaji,*, a
and Anna H. Plaasb
aDepartment of Environmental Health, Faculty of
Pharmaceutical Sciences, Hokuriku University, Ho-3 Kanagawa-machi,
Kanazawa 920-1181, Japan and bDepartment of Internal
Medicine, College of Medicine, University of South Florida,
12901 Bruce B. Downs Blvd., Tampa, FL 33612-4799, U.S.A.
Heparan sulfate (HS) chains bind and activate
fibroblast growth factor-2 (FGF-2) depending on their
microstructure. The glycosaminoglycan chains present
particularly as a large HS proteoglycan perlecan in
vascular endothelial cells. A heavy metal lead induces
a lower response to FGF-2 by inhibition of perlecan
synthesis and inhibits the proliferation of the cells. The
present study was undertaken to address the question
whether lead influences the formation of HS chains in
vascular endothelial cells. The data indicate that lead
decreases the amount of disaccharide units including
hexuronic acid-N-acetylglucosamine, hexuronic
acid-N-sulfated glucosamine and 2-O-sulfated hexuronic
acid-N-sulfated glucosamine in HS chains accumulated
in the cell layer and the conditioned medium.
However, no percentage of any disaccharide unit was
affected by the metal. The percentage of disaccharide
units in chondroitin/dermatan sulfate was also
unaffected by lead. The present data support the
hypothesis that lead-induced lower response of vascular
endothelial cells to endogenous FGF-2 mainly results
from a decrease in perlecan molecules of extracellular
matrix as a result of selective inhibition of perlecan
core protein synthesis rather than a change in the HS
structure.
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