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J.Health Sci., 50(6), 654-659, 2004
Stimulation of Proteoglycan Release from Cultured Vascular Endothelial Cell Layers
by Sodium Spirulan
Toshiyuki Kaji,*, a Satomi
Shimada,a Chika
Yamamoto,a Yasuyuki
Fujiwara,a Jung-Bum
Lee,b and Toshimitsu
Hayashib
aDepartment of Environmental Health, Faculty of Pharmaceutical Sciences, Hokuriku University, Ho-3 Kanagawa-machi,
Kanazawa 920-1181, Japan and bDepartment of Pharmacognosy, Faculty of Pharmaceutical Sciences, Toyama Medical and
Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan
Our previous study suggested that sodium spirulan (Na-SP) stimulated the release of proteoglycans from
cultured vascular endothelial cell layers. In the present study, we characterized the stimulatory effect of Na-SP
on endothelial proteoglycan release from the viewpoint of the Na-SP structure. The following results were
obtained: Na-SP is a potent stimulator of endothelial proteoglycan release. The stimulatory effect of Na-SP
disappears with desulfation or removal of sodium ion. Depolymerized Na-SPs
(Mr ∼14700 or greater) also exhibits
stimulatory effects as does Na-SP
(Mr ∼220000). The stimulation occurs more potently in endothelial cells than
in vascular smooth muscle cells. Therefore it is suggested that the stimulatory effect of Na-SP on proteoglycan
release requires both the sulfate group and sodium ion, and the effect is achieved by an active structure in the
molecule which interacts particularly with the endothelial cell extracellular matrix.
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