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J.Health Sci., 50(6), 634-639, 2004
Characterization of Active Component in Marine
Alga Sargassum horneri Extract in Stimulating
Bone Calcification in Vitro
Satoshi Uchiyama,a Masayuki
Hashizume,b Yoshinori
Hokari,b Taeko
Nakagawa,a
Aki Igarashi,a, c and Masayoshi Yamaguchi*, a
aLaboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka,
52-1 Yada, Shizuoka 422-8526, Japan, bResearch & Development Department, Maruhachi Muramatsu, Inc., 65-1, Jonokoshi, Yaizu,
Shizuoka 425-0025, Japan, and cDepartment of Nutrition, Chukyo Women's University, 55 Nadakayama Yokone-cho, Obu, Aichi
474-8651, Japan
The marine alga Sargassum horneri (S.
horneri) has an anabolic effect on bone metabolism
(J. Health Sci., 47, 533-538, 2001). The effect of the fractionated extracts obtained from
S. horneri on bone calcium content and osteoclast-like cell formation
in vitro was investigated. S.
horneri was gathered from various coasts (Shimoda,
Iwate, or China). Rat femoral-diaphyseal and -metaphyseal tissues were cultured for 24 hr in a medium
containing either vehicle or a water-solubilized extract
(25 mu g/ml of medium) obtained from S.
horneri. Diaphyseal and metaphyseal calcium contents were significantly increased in the presence of
S. horneri extract from Shimoda, Iwate, or China. The active component of
S. horneri extract in increasing calcium content in diaphyseal tissues
was seen in the nearby fraction molecular weight (MW) of 1000. This effect completely disappeared upon heat
treatment with
80°C for 30 min. Mouse marrow cells were cultured for 7 days in a medium containing 1,25-dihydroxyvitamin
D3 [1,25(OH)2D3]
(10-7 M) in the presence or absence of
S. horneri extract (1.0 mu g/ml of medium). The
1,25(OH)2D3-induced increase in osteoclast-like cell formation was markedly suppressed in the presence of the
crude extract or a fraction more than MW 50000 of
S. horneri extracts obtained from Iwate or China. This effect
was also seen by heat treatment. The present study suggests that the active components of
S. horneri extract in stimulating bone formation or suppressing osteoclastic bone resorption are different.
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