| |
Software Requirements
Microsoft Internet Explorer 5.01 or higher and Netscape Navigator 4.75 or higher are recommended. |
|
 |
J.Health Sci., 50(6), 605-612, 2004
Isolation and Characterization of a
1,3-Dichloro-2-Propanol-Degrading Bacterium
Risa Yonetani,a Hisayoshi
Ikatsu,b Chizuko
Miyake-Nakayama,a Eriko
Fujiwara,a
Yoko Maehara,a Shin-ich
Miyoshi,a Hideomi
Matsuoka,c and Sumio
Shinoda*, a
aFaculty of Pharmaceutical Sciences,
bCenter for Environmental Science and Technology, Okayama University, Tsushima-naka,
Okayama 700-8530, Japan, and cSeiko PMC Cooperation, Research and Development Division, Ichihara Laboratory,
Yahatakaigan-dori, Ichihra, Chiba 290-0067, Japan
Arthrobacter sp. strain PY1, a bacterium having the ability to degrade 1,3-dichlotopropanol (1,3-DCP), was
isolated from a soil sample of a chemical plant. Strain PY1 degraded 1000 mg/l (7.75 mM) of 1,3-DCP completely
within 7 days, and the ability was elevated by acclimatization up to 4000 mg/l/week. Addition of nutrients such as
peptone, glucose or glycerol showed no or slight effect on the degrading activity. These results suggest that strain
PY1 is a useful organism in a biological control system for 1,3-DCP pollution. The ability to degrade 1,3-DCP was
induced by addition of 1,3-DCP to the culture of strain PY1. A 1,3-DCP-degrading enzyme (Deh-PY1) was purified
from the cytoplasmic fraction of strain PY1 by fractionation with ammonium sulfate, hydrophobic chromatography
and anion exchange chromatography. Purified Deh-PY1 is a tetramer of a homogeneous subunit having a molecular
weight of 20 kDa. Analysis of the N-terminal amino acid sequence of Deh-PY1 showed that the 31 residues were
quite similar to those of known 1,3-DCP-dehalogenases of other organisms,
Arthrobacter sp. strain AD2 and
Corynebacterium sp. strain N-1074, although some differences in composition or enzymatic characteristics were
observed. The Km value and Vmax of Deh-PY1 were 2.67 mM and
7.81 mu mol/min/mg, respectively, and the
optimum reaction temperature and pH were 40-50°C and 9.5-10.5.
|
|
