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J.Health Sci., 50(6), 600-604, 2004
Preparation of Monoclonal Antibodies Reactive to a Hallucinogenic Drug, Psilocin
Mitsune Yamaguchi,*, a Toshiaki
Saito,b Yoshie
Horiguchi,b Keita
Ogawa,b Yukihiro
Tsuchiya,a Kayoko
Hishinuma,a Toshiyuki
Chikuma,a Yukiko
Makino,c and Hiroshi
Hojoa
Department of aHygienic Chemistry,
bMedicinal Chemistry, Showa Pharmaceutical University, 3-3165 Higashi-tamagawagakuen,
Machida, Tokyo 194-8543, Japan, and cNarcotics Control Department, Kanto-Shin etsu Regional Bureau of Health and Welfare,
Ministry of Health, Labour and Welfare, 2-4-14 Nakameguro, Meguro-ku, Tokyo 153-0061, Japan
The cultivation or trafficking of "Magic mushrooms," containing hallucinogenic psilocin and psilocybin,
has been prohibited by the Narcotics and Psychotropics Control Law in Japan since 2002. To identify these
mushrooms, we attempted to prepare the monoclonal antibody (mAb) reactive to these hallucinogens. As an
antigen inducing an anti-psilocin mAb,
N-{4-[3-(2-dimethylaminoethyl)indol-4-yl-oxy]butyl}succinamic acid
was synthesized by modifying the 4-hydroxyl moiety of psilocin and coupled to a carrier protein of keyhole
limpet hemocyanin. BALB/c mice were immunized five times with the antigen emulsified with an adjuvant, and
their spleen cells were fused with mouse myeloma cells. We obtained several hybridoma cells producing mAbs
reactive to psilocin, from which four clones, BA631, CA231, KA422, and MA332 with a higher production of
anti-psilocin mAb were selected by limiting dilution. Isotype of CA231 and KA422 mAbs were IgG2a and that
of BA631 and MA332 mAbs were IgG1. Enzyme immunoassay (EIA) using BA631 mAb, revealed that BA631
cross-reacted with psilocin and dimethyltryptamine, but not with the other indole derivatives such as psilocybin,
4-hydroxyindole, tryptamine, and tryptophan. Therefore, these antibodies could be used for the identification of
magic mushrooms.
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