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J.Health Sci., 50(6), 594-599, 2004
Spectrofluorometric Determination of Uric
Acid and Glucose by Use of Fe(III)-Thiacalix[4]arenetetrasulfonate
as a Peroxidase Mimic
Junichi Odo,*, a Eri
Shinmoto,a Atsushi
Shiozaki,a Yoko
Hatae,a Seiichi
Katayama,a
and Geng-sheng Jiaob
aDepartment of Biological Chemistry, Faculty of Science, Okayama University of Science, 1-1 Ridai-cho, Okayama 700-0005,
Japan and bDepartment of Chemistry, Weinan Teachers College, Weinan, Shaanxi 714000, China
The peroxidase-like activity of the
Fe3+ complex of thiacalix[4]arenetetrasulfonate on a modified
anion-exchanger
(Fe3+-TCAS[4]A-500) has been applied to the spectrofluorometric determination of uric acid and
glucose in connection with uricase and glucoseoxidase (GOD), respectively. Uric acid and glucose were
spectrofluorometrically determined by measuring the amounts of
H2O2 produced through each catalytic reaction
by uricase and GOD for uric acid and glucose in a sample solution, respectively. The calibration curves obtained
by the
Uricase-Fe3+-TCAS[4]A-500 and the
GOD-Fe3+-TCAS[4]A-500 methods were linear from 0.5 to
5.0 mu g of uric acid in a 1.0 ml sample solution and from 0.5 to
6.0 mu g of glucose in a 0.5 ml sample solution, respectively. The
methods using
Fe3+-TCAS[4]A-500 were applied to the determination of uric acid and glucose in control sera.
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