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J.Health Sci., 50(5), 545-550, 2004
Effects of Perfluoro Organic Compound Toxicity on Nematode Caenorhabditis elegans Fecundity
Nobuaki Tominaga,*, a Shinya
Kohra,b Taisen Iguchi,c, d and Koji
Arizonoc, e
aDepartment of Chemical and Biochemical Engineering, Ariake
National College of Technology, 150 Higashi-hagio-machi,
Omuta 836-8585, Japan, bFaculty of Environmental Sciences,
Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521,
Japan, cCREST, JST, Japan Science and Technology
Corporation, 4-1-8 Honmachi, Kawaguchi, Saitama 332-0012, Japan,
dDepartment of Bio-environmental Research, Center for
Interactive Bioscience, National Institute for Basic Biology, 5-1
Higashiyama myodaiji, Okazaki, Aichi 444-8585, Japan, and
eFaculty of Environmental and Symbiotic Sciences, Kumamoto
Prefectural University, 3-1-100 Tsukide, Kumamoto 862-8502,
Japan
Toxicity studies using the nematode Caenorhabditis elegans
(C. elegans) as a model organism have shown that perfluoro organic compounds
have sub-lethal toxicity at the 10 pM-100 nM range
on multi-generation assays. We examined the acute
lethal toxicity and multi-generational sublethal toxicity
(fecundity and reproduction) of perfluorooctanoic acid
(PFOA), perfluorononanoic acid (PFNA) and salts of
perfluoro-1-octansulfonic acid (PFOS) using 1.7% agar
Nematode Growth Medium (NGM) plates. The fluorine compounds affected the fecundity of
C. elegans at concentrations 105- to
108-fold lower than the median effective concentrations
(EC50). In particular, worm abundance during the first generation did not differ
significantly from controls, while in contrast, the
number of worms in the fourth generation at 10 pM
PFOS-tetraethylammonium (TEA) decreased significantly to about 50% of control (p < 0.01) and the number of eggs and worms in the fourth generation at 1 nM
PFNA decreased significantly to about 30% of control
(p < 0.01). However, no dose-response relationship was
observed in this study. We confirmed that perfluoro
organic compounds strongly disrupt fecundity in
C. elegans.
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