| |
Software Requirements
Microsoft Internet Explorer 5.01 or higher and Netscape Navigator 4.75 or higher are recommended. |
|
 |
J.Health Sci., 50(5), 518-529, 2004
Cloning of Six Full-Length cDNAs Encoding Pig Cytochrome P450 Enzymes and Gene Expression of these Enzymes in the Liver and Kidney
Misaki Kojima*, a and Takeya
Morozumib
aLaboratory of Animal Gene Function, Department of Physiology and Gene Regulation, National Institute of Agrobiological
Sciences, Kannondai 2-1-2, Tsukuba 305-8602, Japan and
bAnimal Genome Research Program Team, STAFF Institute, 446-1
Ippaizuka, Kamiyokoba, Tsukuba 305-0854, Japan
Six full-length cDNAs encoding pig cytochrome P450 (CYP) enzymes, CYP1A1, CYP2A19, CYP2B22,
CYP2C33v4, CYP2C49, and CYP2E1, were isolated and sequenced. The cDNA sequences of pig CYP1A1,
CYP2A19, CYP2B22, CYP2C49, and CYP2E1 showed high similarity to human
CYP1A1 (85.4%), CYP2A13 (88.6%), CYP2B6 (81.1%), CYP2C18 (85.3%), and CYP2E1 (82.5%), respectively, and pig CYP2C33v4 cDNA showed high similarity to rat CYP2C23 (79.2%). Reverse transcription-polymerase chain reaction (RT-PCR)
assays revealed hepatic gene expression of all these pig CYP enzymes: the order of expression was
CYP2C33v4 and CYP2E1 > CYP2C49 > CYP1A1 and CYP2A19 >
CYP2B22. In the kidney, the CYP2C33v4 gene was expressed at the same level as in the liver, but the
CYP1A1, CYP2A19, and CYP2B22 genes were expressed at
lower levels than in the liver. Little renal gene expression of CYP2C49 and CYP2E1 was observed. We revealed
for the first time the full-length cDNA sequences encoding pig CYP1A1 and five CYP enzymes belonging to the
CYP2 gene family, thus making it possible to examine the gene expression levels of these CYP enzymes in pig
tissues by RT-PCR.
|
|
