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J.Health Sci., 50(5), 491-496, 2004
Anabolic Effect of beta-Cryptoxanthin on Bone
Components in the Femoral Tissues of Aged Rats in Vivo and in Vitro
Satoshi Uchiyama,a Takashi
Sumida,b and Masayoshi
Yamaguchi*, a
aLaboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada,
Shizuoka 422-8526, Japan and bResearch & Development, Ehime Beverage Inc., 478 Anjyoji-machi, Matsuyama 791-8603, Japan
The effect of beta-cryptoxanthin on bone components in the femoral tissues of aged rats was investigated.
beta-Cryptoxanthin was isolated from Satsuma mandarin
(Citrus unshiu MARC.). beta-Cryptoxanthin (5, 10, or
20 mu g/100 g body weight) was orally administered once daily for 7 days to aged (50 week old) female rats. The administration of
beta-cryptoxanthin (10 or 20 mu g/100 g body weight) caused a significant increase in calcium content, alkaline
phosphatase activity and deoxyribonucleic acid (DNA) content in the femoral-diaphyseal and -metaphyseal tissues.
Femoral-diaphyseal calcium content and alkaline phosphatase activity were significantly increased by the dose of
5 mu g/100 g body weight. A significant increase in metaphyseal alkaline phosphatase activity was also seen with the dose
of 5 mu g beta-cryptoxanthin/100 g body weight. Moreover, bone tissues were cultured for 48 hr in serum-free Dulbecco's
modified Eagle's medium containing either vehicle or
beta-cryptoxanthin (10-7 or
10-6 M). The presence of
beta-cryptoxanthin (10-7 or
10-6 M) caused a significant increase in calcium content and alkaline phosphatase activity in the
femoral-diaphyseal and -metaphyseal tissues obtained from aged (50 week old) female rats. These increases were
completely abolished in the presence of cycloheximide
(10-6 M), an inhibitor of protein synthesis. This study
demonstrated that beta-cryptoxanthin has an anabolic effect on bone components in aged female rats
in vivo and in vitro.
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