| |
Software Requirements
Microsoft Internet Explorer 5.01 or higher and Netscape Navigator 4.75 or higher are recommended. |
|
 |
J.Health Sci., 50(5), 483-490, 2004
Purification of Active Component in Wasabi Leafstalk
(Wasabia japonica MATSUM.) Extract in Stimulating Bone Calcification
in Vitro
Toshihiro Suzukia and Masayoshi
Yamaguchi*, b
aFunctional Food Project Team, Shizuoka Industrial Research Institute of Shizuoka Prefecture, 2078 Makigaya, Shizuoka 421-1298,
Japan and bLaboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka,
52-1 Yada, Shizuoka 422-8526, Japan
The active component in wasabi leafstalk (Wasabia japonica
MATSUM.), which has a stimulatory effect on
bone calcification in mouse calvaria tissue culture
in vitro, was purified. Wasabi leafstalk extract was obtained from
a homogenate with 20% ethanol. The active component, which was found in ethanol extraction, was purified by gel
filtration chromatography with HiLoad 26/80 Superdex 30 pg column and reversed-phase chromatography (RPC)
on a resource 15 RPC 3 ml column. The result of ESI mass spectra of the purified active component showed that its
material had a molecular weight of 158. The absorption spectra of the material gave the maximum absorption at a
wavelength of 221 nm. The material in wasabi leafstalk extract differed from genistein and
17beta-estradiol, which can stimulate bone calcification
in vitro, and had a comparatively lower molecular weight. This study demonstrates that
the material with a low molecular weight of 158 is an active component in wasabi leafstalk which stimulates bone
calcification.
|
|
