| |
Software Requirements
Microsoft Internet Explorer 5.01 or higher and Netscape Navigator 4.75 or higher are recommended. |
|
 |
J.Health Sci., 50(3), 301-308, 2004
Validation of an Enzyme-Linked Immunosorbent Assay Method for
Vitellogenin in the Medaka
Norihisa Tatarazako,*, a,
b Masaaki Koshio,a Hiroyuki
Hori,c Masatoshi
Morita,a and Taisen Iguchib, d
aNational Institute for Environmental Studies, 16-2 Onogawa,
Tsukuba, Ibaraki 305-8506, Japan, bCREST, JST (Core
Research for Evolutional Science and Technology, Japan Science
and Technology Corporation), 3-13-11 Shibuya, Shibuya-ku,
Tokyo 150-0002, Japan, cEnvironmental Health and Safety
Division, Environmental Health Department, Ministry of the
Environment, Japan, 1-2-2, Kasumigaseki, Chiyoda-ku, Tokyo
100-8975, Japan, and dCenter for Integrative Bioscience,
Okazaki National Research Institutes, 5-1 Higashiyama,
Myodaiji, Okazaki, Aichi 444-8585, Japan
An enzyme-linked immunosorbent assay (ELISA)
was validated for the measurement of vitellogenin (VTG) in the cyprinid model species, medaka
(Oryzias latipes). Both polyclonal antibody- and monoclonal
antibody-based kits showed very high correlation
between VTG values in unknown samples when we used
standardized VTG protein. This was confirmed by 5 laboratories which participated in the ring test. The
use of one gold standard VTG is essential to measure
VTG values using ELISA kits in future OECD ring tests to screen for estrogenic chemicals in the 3 fish
species medaka, zebrafish and fathead minnow.
|
|
