| |
Software Requirements
Microsoft Internet Explorer 5.01 or higher and Netscape Navigator 4.75 or higher are recommended. |
|
 |
J.Health Sci., 50(2), 126-132, 2004
Sensitive Detection of Bacteria and Spores Using a Portable
Bioluminescence ATP Measurement Assay System Distinguishing
from White
Powder Materials
Yoshihito Fujinami, Mieko Kataoka, Koji Matsushita, Hiroyuki Sekiguchi, Teruo
Itoi, Kouichiro Tsuge, and Yasuo Seto*
National Research Institute of Police Science, 6-3-1
Kashiwanoha, Kashiwa, Chiba 277-0882, Japan
The CheckLite™ 250 Plus portable bioluminescence assay system for measuring bacterial
adenosine 5'-triphosphate (ATP) was investigated for its
performance with respect to the field detection of
bacteria in bioterrorism incidents. Vegetative bacteria,
Escherichia coli (E. coli), Bacillus
subtilis (B. subtilis), Staphylococcus
aureus, and Yersinia
pseudotuberculosis gave considerably high
luminescence in a dose-dependent manner with responses
of 104-105 relative light units (RLU) per
107 cells, whereas spore forms of B.
subtilis showed considerably low luminescence with 214 RLU per
109 spores. Typical white powder materials such as wheat flour,
sugar, and bovine serum albumin at concentrations
of 0.1 and 0.5% (w/v) gave only negligible
luminescence (lower than 400 RLU), and did not change the
luminescence of E. coli cells significantly (42-102%
of the control). The luminescence of B.
subtilis spores increased considerably to over
104 RLU per 107 spores by pretreatment consisting of 37°C incubation for
30 min in nutrient broth medium containing 4 mM
L-alanine. The increased luminescence by this
pretreatment was not changed considerably (42-145%
of the control) in the presence of the above tested
white powder materials (0.1 and 0.5%).
|
|
