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J.Health Sci., 49(6), 531-533, 2003
Fungal Identification Method Based on DNA Sequence Analysis: Reassessment of the Methods of the Pharmaceutical Society of Japan and the Japanese Pharmacopoeia
Takashi Sugita*, a and Akemi Nishikawab
aDepartment of Microbiology and bDepartment of Immunobiology, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan
Fungal identification methods based on DNA sequence analysis were recently published in Methods of Analysis in the Health Sciences (Eisei-shiken-ho in Japanese) issued by the Pharmaceutical Society of Japan (PSJ) and in the draft second supplement to the 14th edition of the Japanese Pharmacopoeia (JP). We carefully compared and assessed the effectiveness of the two methods of fungal identification. The PSJ method analyzes D1/D2 26S rDNA, while the JP method analyzes the internal transcribed spacer (ITS) 1 region. The former method is superior to the latter in that the identification criterion described in the PSJ method is rationally defined based on the species concept, whereas distinct species can be misidentified as being of the same species using the identification criterion described in the JP method; and there are more sequence data for the D1/D2 26S rDNA regions in the DNA data libraries (DDBJ/GenBank/EMBL) than for ITS1 data. Based on our assessment, we conclude that the PSJ method is superior to the JP method for fungal identification.
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