| |
Software Requirements
Microsoft Internet Explorer 5.01 or higher and Netscape Navigator 4.75 or higher are recommended. |
|
 |
J.Health Sci., 49(6), 514-519, 2003
Organotin Compounds Suppress Testosterone Production in Leydig Cells from Neonatal Pig Testes
Yonako Nakajima, Go Sato, Shuji Ohno,
and Shizuo Nakajin*
Department of Biochemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan
The in vitro effects of exposure to tributyltin chloride (TBT), dibutyltin dichloride (DBT), and triphenyltin chloride (TPT) on testosterone production in isolated Leydig cells from neonatal pig testes were examined. These organotin compounds strongly suppressed human chorionic gonadotropin (10 IU/ml) or 8-bromo-cAMP (0.1 mM) induced testosterone production when the cells were exposed to nontoxic concentrations. The suppression of testosterone production was dose dependent over the concentration range of 0.03-0.3 mu M for TBT and DBT, and 0.01-0.3 mu M for TPT. These effective concentrations for the suppression of testosterone production were lower than that of the cytotoxic concentrations of the organotin compounds in Leydig cells and were equivalent to the accumulated concentration levels that were previously reported in marine organisms.
|
|
