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J.Health Sci., 49(5), 401-404, 2003
Identification of Oil-Soluble Coal Tar Dyes in Cosmetics Using Reversed-Phase TLC/Scanning Densitometry
Tsutomu Ohno,* Eiichi Mikami, and Hiroshi Matsumoto
Aichi Prefectural Institute of Public Health, Nagare 7-6, Tsuji-machi, Kita-ku, Nagoya 462-8576, Japan
A simple and rapid analytical method of oil-soluble coal tar dyes in cosmetics was established using reversed-phase TLC/scanning densitometry. Eleven kinds of oil-soluble coal tar dyes were able to be separated completely on reversed-phase TLC plates by the complementary use of 2 solvent systems. The solvent systems were A; n-hexane/2-butanone solution (5 : 1, v/v), solvent system B; acetonitrile/methanol solution (5 : 1, v/v). Then we measured visible absorption spectra of spots developed on the reversed-phase TLC plates by scanning densitometer to identify these coal tar dyes. The proposed method was successfully applied to the identification of oil-soluble coal tar dyes in commercial cosmetics.
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