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J.Health Sci., 49(5), 386-390, 2003
Determination of the Mutagen 3-Nitrobenzanthrone in Rainwater Collected in Kyoto, Japan
Tsuyoshi Murahashi,* Erina Iwanaga, Tetsushi Watanabe, and Teruhisa Hirayama
Kyoto Pharmaceutical University, 5 Nakauchi-cho Misasagi Yamashina-ku, Kyoto 607-8414, Japan
The mutagen 3-nitrobenzanthrone was determined in rainwater. Rainwater was collected at a residential area in Kyoto, Japan. Organic matter containing 3-nitrobenzanthrone in rainwater was extracted using a 3M (St. Paul, MN, U.S.A.) Empore Extraction Disk C18, and the extract was purified on a pyrenylethyl silylated silica gel column. The purified sample was then reduced using a platinum black column heated at 80°C. The reduced product of 3-nitrobenzanthrone, i.e. 3-aminobenzanthrone, was analyzed by normal-phase HPLC with fluorescence detection. 3-Nitrobenzanthrone in the rainwater was determined in the range of 0.07-2.6 ng l-1. The concentration was higher from May to July and lower from October to December. 1-Nitropyrene was also determined by reversed-phase HPLC with fluorescence detection after platinum black column reduction. 1-Nitropyrene was detected in the range of 0.0056-0.19 ng l-1, and this concentration range was lower than that of 3-nitrobenzanthrone.
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