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J.Health Sci., 49(4), 311-315, 2003
Novel Bioassay for the Assessment of Neurotoxicity of Chemicals Based on the Neurite
Extension in Human Neuroblastoma NB-1 Cells
Manabu Kunimoto,*, a Risa
Yoshimi,a Sachiko
Matsushita,a Motoharu
Sakaue,a Hiromi
Takanaga,a Shuntaro
Hara,a Hideo
Utsumi,b and Osami
Nakasugic
aDepartment of Public Health, School of Pharmaceutical Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo
108-8641, Japan, bLaboratory of Bio-function Analysis, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1
Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, and
cChemical Risk Assessment Center, National Institute for Environmental
Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan
A new testing method for the assessment of hazardous environmental chemicals using cultured human
neuronal cells has been developed. This method is based on the image analysis of the neurite extension in human
neuroblastoma NB-1 cells: the length of the extended neurites was determined using image analysis software.
Using this system, 255 chemicals including methylmercury and endocrine disrupting chemicals were tested.
Methylmercury and several chemicals inhibited neurite extension, while cadmium chloride, phthalates, and
many other chemicals promoted it. These results suggest that the extended neurite length is a useful biological
marker for the effects of neurotoxic environmental chemicals, especially on the developing nervous system.
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