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J.Health Sci., 49(2), 123-128, 2003
Anabolic Effect of Wasabi Leafstalk (Wasabia
japonica MATSUM.) Extract on Bone Components in the Femoral-Diaphyseal and
-Metaphyseal Tissues of Aged Female Rats in
Vitro and in Vivo
Masayoshi Yamaguchi,*, a Zhong Jie
Ma,a and Toshihiro
Suzukib
aLaboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka,
52-1 Yada, Shizuoka 422-8526, Japan and
bFunctional Food Project Team, Shizuoka Industrial Research Institute of Shizuoka
Prefecture, 2078 Makigaya, Shizuoka 421-1298, Japan
The effect of wasabi leafstalk (Wasabia
japonica MATTUM.) extract on bone components in the
femoral-diaphyseal and -metaphyseal tissues of aged female rats
in vitro and in vivo was investigated.
Femoral-diaphyseal and -metaphyseal tissues were cultured for 48 hr in Dulbecco's modified Eagle's medium (serum free)
containing either vehicle or wasabi leafstalk extract (10, 25 or 50
mu g/ml of medium). The presence of wasabi leafstalk extract (50
mu g/ml) caused a significant increase in calcium content, alkaline phosphatase activity and
deoxyribonucleic acid (DNA) content in the diaphyseal and metaphyseal tissues
in vitro. However, the effect of wasabi leafstalk extract (50
mu g/ml) in increasing bone components was completely abolished in the presence of
cycloheximide (10-6 M), an inhibitor of protein synthesis. Moreover, rats were orally administered wasabi
leafstalk extract (10 or 20 mg/100 g body weight) once daily for 7 days. The calcium content, alkaline phosphatase
activity and DNA content in the femoral-diaphyseal and -metaphyseal tissues of aged rats was significantly
increased by the administration of wasabi leafstalk extract (10 or 20 mg/100 g) for 7 days
in vivo. Meanwhile, body weight, serum calcium and inorganic phosphorus concentrations of female aged rats were not significantly
altered by the administration of wasabi leafstalk extract (10 or 20 mg/100 g) for 7 days. The present study
demonstrates that wasabi leafstalk extract has an anabolic effect on bone components
in vitro and in vivo. The intake of wasabi leafstalk extract may have a preventive effect on bone loss with increasing age.
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