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J.Health Sci., 49(2), 115-122, 2003
Vascular Smooth Muscle Cells in Culture Express Tumor Necrosis
Factor-alpha That Suppresses Collagen Synthesis Depending on Cell Density
Toshiyuki Kaji,*, a Syouichi
Hiraga,a Chika
Yamamoto,a Yasuyuki
Fujiwara,a Yoshimichi
Ueda,b Fumiko
Zisaki,b Kazushi
Iwata,c Shogo
Katsuda,b and Isao
Nakanishid
aDepartment of Environmental Health, Faculty of Pharmaceutical Sciences, Hokuriku University, Ho-3 Kanagawa-machi,
Kanazawa 920-1181, Japan, bDepartment of Pathology, Kanazawa Medical University, 1-1 Daigaku, Uchinada-machi,
Kahoku-gun, Ishikawa 920-0293, Japan,
cBiopharmaceutical Department, Fuji Chemical Industries, Ltd., 530 Chokeiji, Takaoka, Toyama
933-8511, Japan, and dDepartment of Pathology, School of Medicine, Kanazawa University, 13-1, Takara-machi, Kanazawa
920-8640, Japan
Tumor necrosis factor-alpha (TNF-alpha) has been implicated in the pathogenesis of atherosclerosis through
influence on vascular smooth muscle cell behavior. However, little is known about the expression and function of
endogenous TNF-alpha in the cells. Dense and sparse cultures of bovine aortic smooth muscle cells were prepared
and the expression of TNF-alpha and regulation of collagen synthesis by endogenous
TNF-alpha were investigated. The results indicate that dense and sparse vascular smooth muscle cells express endogenous
TNF-alpha that suppresses the synthesis of collagen (types I and III) only when the cell density is high; suppression was also observed in
human and rabbit aortic smooth muscle cells. Transforming growth
factor-beta stimulated collagen synthesis in bovine aortic smooth muscle cells and the stimulation was significantly suppressed by endogenous
TNF-alpha when cell density was high. The present data suggest that vascular smooth muscle cells express endogenous
TNF-alpha that is involved in the suppressive regulation of collagen synthesis depending on the cell density.
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