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J.Health Sci., 49(1), 8-12, 2003
Biological Evaluation of the Pollution of the Tsurumi River with 7-Ethoxycoumarin
O-Deethylase Activity Induced by River Sediment
Extracts in HepG2 Cells
Shin'ichi Nito,* Yuichiro Kanno, Akiko Muto, Akinori Uesugi, Takayuki Nakahama, and Yoshio Inouye
School of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi-shi, Chiba 274-8510, Japan
The pollution level of the Tsurumi River flowing into Tokyo Bay was studied longitudinally using river sediment extracts in an assay system based on the arylhydrocarbon receptor (AhR)-dependent induction of 7-ethoxycoumarin O-deethylase (ECOD) activity in HepG2 cells. The sampling points of river sediment were as follows: Namamugi, Kami-Sueyoshi, Tsunashima, Shin-Yokohama A and B (downstream and upstream, respectively, from the former open-air industrial waste incineration site), Kozukue and Nakayama (Fig. 1). ECOD activity was induced to different extents in all samples tested. Namamugi, located in the middle of Keihin Industrial District, was considered to be the most polluted, followed by Shin-Yokohama A and Tsunashima. Results from these samples showed reverse U-shaped dose-response curves in terms of ECOD activity as well as 3-methylcholantrene (3-MC) although the expression of CYP1A1 mRNA in HepG2 cells remained roughly constant at higher concentrations as in the case of 3-MC, thus implying post-transcriptional suppression.
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