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J.Health Sci., 48(6), 503-513, 2002
Possible Origin of Rat Testicular Atrophy Induced by Di-n-Butyl Phthalate: Changes in the Activities of Some Enzymes during Rat Testis Perfusion under a Hypoxic Condition and with Mono-n-Butyl Phthalate
Nobuo Watanabe, Makiko Shimizu, Yoshiaki Matsumoto, and Masamichi Fukuoka*
Department of Clinical Pharmacology and Toxicology, Showa Pharmaceutical University, Higashi-Tamagawagakuen 3-3165, Machida-city, Tokyo 194-8543, Japan
To examine whether testicular toxicity in rats is caused by a direct effect of mono-butyl phthalate (MBP), a metabolite of di-butyl phthalate, or by a secondary effect attributed to a hypoxic condition due to the MBP-induced hemoglobin deprivation, the testes were perfused with a solution of MBP in Eagle's MEM or the MEM with/without oxygen, and the activities of testicular enzymes were measured. A decrease in the succinate dehydrogenase (SUDH) activity was observed by the hypoxic perfusate [20-30% dissolved oxygen (DO)], and an induction of apoptosis was observed by the 7% DO perfusate. However, the 100mM MBP perfusate decreased the activity of SUDH per testis weight, but not per protein level. Therefore, this study proposes that the toxicity might be caused by hypoxia and a coincident depletion of SUDH activity, followed by an apoptotic testicular cell death.
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