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J.Health Sci., 48(5), 455-459, 2002
Effect of Estrogenic Compounds on Superoxide and Nitric Oxide Production by Activated Macrophages Assessed by Sensitive Microplate Assays
Wataru Yoshida, Minori Ogawa, Yuko Furuoya, Yu Igarashi, Rumi Sato, Akiko Hayashi, Kazuya Hirano, and Masatoshi Beppu*
Laboratory of Public Health, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan
The effect of estrogenic compounds (bisphenol A, genistein, and 17beta-estradiol) on the ability of macrophages to produce superoxide and nitric oxide in response to stimulants was investigated. The assays were performed using 96-well microplates to measure multiple samples. Superoxide and nitric oxide were measured by chemiluminescence and fluorescence methods, respectively. Thioglycollate-induced mouse peritoneal macrophages incubated for 24 hr with the estrogenic compounds were stimulated with phorbol 12-myristate 13-acetate and lipopolysaccharide (LPS). The ability of the macrophages to produce superoxide was increased when treated with bisphenol A or genistein, but 17beta-estradiol had little effect. Conversely, the ability of the macrophages to produce nitric oxide in response to LPS was strongly suppressed by high concentrations of bispenol A and genistein. 17beta-estradiol was ineffective. These results suggest that bisphenol A and genistein similarly affect superoxide and nitric oxide production by a mechanism other than that involving estrogenic activity.
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