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J.Health Sci., 48(5), 441-445, 2002
Sensitivities of mRNA Expression of Vitellogenin, Choriogenin and Estrogen Receptor by Estrogenic Chemicals in Medaka, Oryzias latipes
Chulwoo Lee,a Seong Hwan Jeon,a Jin-Gyun Na,a Yong-Jin Choi,b and Kwangsik Park*, a
aRisk Research Department, National Institute of Environmental Research, Gyeongseo-dong, Seo-gu, Incheon 404-170, Korea and bLaboratory of Microbial Genetics, Graduate School of Biotechnology, Korea University, #5-1, Anam-dong, Seongbuk-gu, Seoul 136-701, Korea
Gene expression levels of choriogenin, vitellogenin, and estrogen receptor were determined using a reverse transcription-polymerase chain reaction (RT-PCR) technique after exposure to estrogenic chemicals to compare the sensitivities of the biomarkers of endocrine disruption in medaka, Oryzias latipes. Mature male medaka were treated with a single dose of 100 mu/l of 17alpha-ethinylestradiol, nonylphenol, and bisphenol A for 6 days, then RNA was extracted from the livers of treated fish for RT-PCR. Primers of RT-PCR for choriogenin H and L, and estrogen receptor were synthesized based on previously known cDNA sequences, and primers for vitellogenin I and II were synthesized based on the partial cDNA which was sequenced in this study. When the five biomarker genes were amplified by RT-PCR under the same condition, the mRNA induction level of each gene was elevated with different sensitivities. Conclusively, choriogenin L, which is a precursor of zona radiata protein (ZI-3) with molecular weight of 49 kD, showed the most sensitive gene expression in all the treated groups.
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