| |
Software Requirements
Microsoft Internet Explorer 5.01 or higher and Netscape Navigator 4.75 or higher are recommended. |
|
 |
J.Health Sci., 48(5), 412-417, 2002
Analysis of Aristolochic Acid I and II in Kampo Medicine Preparations
Takako Seto,* Tomoko Hamano, Hiroko Shioda, and Hisashi Kamimura
Tokyo Metropolitan Research Laboratory of Public Health, 24-1 Hyakunin-cho 3-chome Shinjuku-ku, Tokyo 169-0073, Japan
Herein reported are the detection methods of Aristolochic acid I (AA-I) and aristolochic acid II (AA-II), nephrotoxic components of the Aristolochia plants in Aristolochiaceae, in the Kampo medicine Toki-shigyaku-ka-goshuyu-shokyo-to. The confirmation of AA-I and AA-II was possible by the detection methods using a thin layer chromatography and a high performance liquid chromatography equipped with a Photodiode array detector (LC-PDA) and an electric ionization mass spectrometry detector. With the LC-PDA, it was possible to quickly detect AA-I and AA-II in the ten kinds of Kampo medicines in which the Aristolochia fangchi root or Aristolochia mandshuriensis stem may be misused. By using AA-I and AA-II purified from a commercial mixture by a preparative HPLC as reference standards, quantitative analyses of AA-I and AA-II were successfully carried out by use of the LC-PDA.
|
|
