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J.Health Sci., 48(4), 381-384, 2002
Methylmercury-Mediated Down-Regulation of mtHSP70 and Phospholipase A2 mRNA Expression in Human Neuroblastoma NB-1 Cells Identified by cDNA Macroarray Analysis
Rocky Pramanik,a, b Masami Ishido,a, b and Manabu Kunimoto*, a, b
aRegional Environment Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba 305-8506, Japan and bCREST, Japan Science and Technology (JST) Cooperation, Kawaguchi, Saitama 332-0012, Japan
Methylmercury (MeHg) is a well-known environmental pollutant that causes severe neurological damage, especially in the developing nervous system. When human neuroblastoma NB-1 cells were treated with MeHg at sublethal concentrations, down-regulation of two apoptosis-associated genes, mtHSP70 (GRP75/PBP74/ mtHSP75 /HSPA9B/mortalin) and phospholipase A2 (PLA2), was identified using the cDNA macroarray technique. These observations were further confirmed by RT-PCR and Northern blotting experiments. Northern blotting data also demonstrated that only groups III and VI B of PLA2 were down-regulated, while group IIE remained unchanged. These results suggest that the mtHSP70 and PLA2 may be involved in the MeHg-mediated degeneration of neuronal cells.
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