| |
Software Requirements
Microsoft Internet Explorer 5.01 or higher and Netscape Navigator 4.75 or higher are recommended. |
|
 |
J.Health Sci., 48(4), 303-309, 2002
Induction of Apoptosis with an Extract of Actinidia polygama Fruit in the Promyelocytic Leukemia Cell Line HL-60
Yuko Yoshizawa,a Yoshihiro Fukiya,b Yoshikatsu Izumi,b Keishi Hata,c Jun Iwashita,b Noboru Murofushi,a and Tatsuya Abe*, b
aBio-organic Chemistry and bMolecular Biology, Akita Prefectural University, Shimoshinjo Nakano, Akita City, Akita 010-0195, Japan, and cAkita Research Institute of Food & Brewing, 4-26 Sanuki, Araya-machi, Akita 010-1623, Japan
Common small fruit produced naturally in Japan is attracted as healthy food. We studied on inhibitory activity of several small fruit extracts on growth of cancer cells to look for a candidate of healthy food or low toxic anti-cancer drugs. Among the fruit extracts examined, Actinidia polygama (Matatabi) extract had dose-dependent potent inhibitory activity against the growth of a promyelocytic leukemia cell line, HL-60, but was less effective on a colon cancer cell line, LS-174T, or normal human dermal fibroblast cells. We examined a part of the responsible mechanism of growth inhibition of HL-60 cells. Active agents in A. polygama fruit were heat-stable and small molecular weight of less than 1000. HL-60 cells incubated with an extract of A. polygama fruit induced apoptosis, which was detected by electrophoretic DNA fragmentation, morphological chromatin condensation and the TUNEL method with flow cytometry.
|
|
