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J.Health Sci., 48(3), 269-272, 2002
A Rapid Respiratory Toxicity Test Using Caenorhabditis elegans with an Oxygen Electrode System
Shinya Kohra,*, a Nobuaki Tominaga,b Yuji Takao,a Masaki Nagae,a Yasuhiro Ishibashi,c Kazuo Ueda,a
and Koji Arizonod
aFaculty of Environmental Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan, bDepartment of Chemical and Biological Engineering, Ariake National College of Technology, 150 Higashihagino-machi, Omuta 836-8585, Japan, cEnvironmental Protection Center, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan, and dFaculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, 3-1-100 Tsukide, Kumamoto 862-8502, Japan
We describe a novel approach to evaluating the respiratory toxicity of chemicals in the free-living nematode Caenorhabditis elegans. Using DOX-96KT, a general purpose, multi-channel dissolved oxygen (DO) measuring system, we measured the DO concentration in culture media containing C. elegans exposed to chemicals to assay for respiratory toxicity. The current value, which is an index of the dissolved oxygen concentration in culture media, was measured every 10 sec for 30 min at 24°C. We focused on the respiration levels of the exposed worms between 500 and 1800 sec. This method produces results that are similar to the computer tracking system measuring behavioral toxicity. Since it can do multiple dilution series tests at a given time, it is useful for concentration-activity correlation studies. This novel technique is not only an alternative to the computer tracking system for measuring behavioral toxicity but also a rapid sublethal toxicity test for chemical hazard assessment.
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