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J.Health Sci., 48(2), 154-160, 2002
Inhibitory Effect of Marine Alga Sargassum horneri Extract on Bone Resorption in Tissue Culture in Vitro
Satoshi Uchiyama and Masayoshi Yamaguchi*
Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan
The effect of marine alga Sargassum horneri extracts on bone resorption in vitro was investigated. Femoral-diaphyseal and -metaphyseal tissues obtained from normal rats were cultured for 48 hr in Dulbecco's modified Eagle's medium (high glucose, 4.5%) supplemented with antibiotics and bovine serum albumin. The experimental cultures contained water-solubilized extracts (10, 25, and 50 mu g/ml of medium) of S. horneri. The bone-resorbing factors parathyroid hormone (1-34) (PTH; 10-7 M) and prostaglandin E2 (PGE2; 10-5 M) caused a significant decrease in the diaphyseal and metaphyseal calcium content. The decrease in bone calcium content induced by PTH or PGE2 was completely inhibited by water-solubilized extracts (10, 25, and 50 mu g/ml) of S. horneri. In addition, these extracts (25 and 50 mu g/ml) completely prevented the PTH (10-7 M)- or PGE2 (10-5 M)-induced increase in medium glucose consumption and lactic acid production by bone tissues. Moreover, S. horneri extracts (10-50 mu g/ml) blocked PTH (10-7 M)- increased acid phosphatase activity in the diaphyseal and metaphyseal tissues. These findings indicate that water-solubilized extracts of S. horneri have a direct inhibitory effect on bone resorption in tissue culture in vitro.
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