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J.Health Sci., 47(6), 583-586, 2001
Effect of Dominant-Negative Expression of L1 Cytoplasmic Domain on the Localization of Brain Ankyrins in Human Neuroblastoma NB-1 Cells
Jin-Yi Han, Tatsumi Adachi, Masami Ishido, and Manabu Kunimoto*
Regional Environment Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-0053, Japan
Two isoforms of brain ankyrin, 440 kD and 220 kD ankyrinB, and neuronal cell adhesion molecule L1, which can bind to brain ankyrin, are both expressed in human neuroblastoma NB-1 cells. To examine the potential importance of the interaction between brain ankyrins and L1 in neuronal cell functions, an expression vector encoding a cytoplasmic domain of L1 has been constructed and transfected in NB-1 cells. The results obtained show that the expressed L1 cytoplasmic domain is ineffective in perturbing the interaction between brain ankyrins and L1, possibly because it exists in a monomeric form.
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