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J.Health Sci., 47(4), 424-428, 2001
Detection of Biotin-Binding Immunoglobulin G in Human Sera Using Avidin-Coated Multiwell Microplates
Makoto Muratsugu,*, a Kazunari Kumasaka,b Mami Tanaka,a Keiko Okushima,a
and Toru Fukuic
aDepartment of Bioanalytical Science, Osaka Prefecture College of Health Sciences, Habikino, Osaka 583-8555, Japan, bDepartment of Clinical Pathology, Nihon University School of Medicine, Itabashi, Tokyo 173-8610, Japan, and cByotai-Seiri Laboratory, Itabashi, Tokyo 173-0025, Japan
Biotin-binding human immunoglobulin G (B-IgG) was detected by two different methods in the avidin-coated multiwell format for the first time. In the first method, B-IgG was caught by avidin supported on the solid phase and was detected by alkaline phosphatase (ALP)-labeled anti-human IgG (method A). This method, however, was not able to distinguish B-IgG-positive and -negative human sera. The second method included peroxidase-labeled avidin instead of ALP-labeled anti-human IgG (method B). The sensitivity of method B was 28 times higher than that of method A. Method B in the multiwell-microplate format could detect B-IgG in the IgG fraction purified from human sera for the first time. This suggests that the level of B-IgG in human sera is very low.
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